Introduction:

1. DNA is unique for everyone. The only exception is if a person has what?
If that person has an identical twin.

2. What are DNA fingerprints used for?
It can be used to figure out biological relatives or solve a crime.

Part 1 “It Takes a Lickin”

3. What “crime” was committed?
A holographic NOVA lollipop belonging to Johnny was partly eaten.

4. What bodily fluid was removed from the “crime scene” to get DNA?
Saliva

Part 2 “DNA Fingerprinting at the NOVA Lab”

5. What does a restriction enzyme do?
Restriction enzymes split the DNA in different places.

6. What is agarose gel?
A molecular strainer for the larger DNA.

7. What is electrophoresis?
An electrophoresis machine moves molecules electricity.

8. Smaller fragments of DNA move ____________ than longer strands?
Faster.

9. Why do you need to place a nylon membrane over the gel?
The nylon membrane is needed because it is difficult to pick up the gel
without special equipment.


10. Probes attach themselves to __________
Probes attach to the DNA fragments on the nylon membrane.

11. Which chemical in your “virtual lab” is radioactive?
The probes are radioactive.

12. Sketch your DNA fingerprint.
It was a column of lines.


13. Based on your DNA fingerprint, who licked the lollipop?
Honey sweet is the thief.