Introduction:

1. DNA is unique for everyone. The only exception is if a person has what?
If that person has an identical twin.

2. What are DNA fingerprints used for?
It can be used to figure out biological relatives or solve a crime.

Part 1 “It Takes a Lickin”

3. What “crime” was committed?
A holographic NOVA lollipop belonging to Johnny was partly eaten.

4. What bodily fluid was removed from the “crime scene” to get DNA?
Saliva

Part 2 “DNA Fingerprinting at the NOVA Lab”

5. What does a restriction enzyme do?
Restriction enzymes split the DNA in different places.

6. What is agarose gel?
A molecular strainer for the larger DNA.

7. What is electrophoresis?
An electrophoresis machine moves molecules electricity.

8. Smaller fragments of DNA move ____________ than longer strands?
Faster.

9. Why do you need to place a nylon membrane over the gel?
The nylon membrane is needed because it is difficult to pick up the gel
without special equipment.


10. Probes attach themselves to __________
Probes attach to the DNA fragments on the nylon membrane.

11. Which chemical in your “virtual lab” is radioactive?
The probes are radioactive.

12. Sketch your DNA fingerprint.
It was a column of lines.


13. Based on your DNA fingerprint, who licked the lollipop?
Honey sweet is the thief.

Mitosis

Mitosis Tutorial
http://www.cellsalive.com/

1. Which stage does the following occur

Chromatin condenses into chromosomes

Prophase

Chromosomes align in center of cell.

Metaphase

Longest part of the cell cycle.

Interphase

Nuclear envelope breaks down.

Prometaphase

Cell is cleaved into two new daughter cells.

Cytokinesis

Daughter chromosomes arrive at the poles.

Telephase


Watch the video carefully.

2. The colored chromosomes represent chromatids. There are two of each color because one is an exact duplicate of the other.

--How many chromosomes are visible at the beginning of mitosis? 4

-- How many are in each daughter cell at the end of mitosis? 4

--The little green T shaped things on the cell are: Centrioles

-- What happens to the centrioles during mitosis? They move to the poles of the parent cell.

3 . Identify the stages of these cells:

a) Metaphase

b) Interphase

c) Telephase

Another Mitosis Animation
Go to www.johnkyrk.com/mitosis.html

View the animation and sketch the cell in:


Onion Root Tip - Online Activity
http://www.biology.arizona.edu/cell_bio/activities/cell_cycle/cell_cycle.html

Read the introduction, then click the “next” button.

You will have 36 cells to classify. When you’re finished, record your data in the chart below.

Interphase Prophase Metaphase Anaphase Telophase Total


Number of cells 20 10 3 2 1 36


Percent of cells 55% 28% 8% 6% 3% 100%

Mitosis in Whitefish & Onion Roots
View 1 View 2 View 3 View 4 View 5


Whitefish Telophase Metaphase Prophase Anaphase


Onion Prophase Metaphase Interphase Telophase Anaphase